Composite
IBR-T25

Part:BBa_K3254004

Designed by: Yeqing Zong, Qianrui Li   Group: iGEM19_GENAS_China   (2019-10-13)
Revision as of 03:42, 14 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


Int10attB-BsaI site-terminator-Int10attP

We expected that this part can be placed between a promoter and a translational unit part and work as a normally open (NO) switch for the downstreamed gene. and switch to ON state by excising the terminator ECK120020525 between the att sites when it reacted with Int10 integrase (BBa_K3254009). Two BsaI restriction site were added between attB site and terminator. As a result, it can work as a normally closed (NC) switch for the gene which was inserted between the two BsaI site and switch to OFF state when it was excised.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 76
    Illegal BsaI.rc site found at 64


[edit]
Categories
//function/recombination
Parameters
None