Composite
IBR-S37

Part:BBa_K3254002

Designed by: Yeqing Zong, Yuqing Yang   Group: iGEM19_GENAS_China   (2019-10-13)
Revision as of 03:20, 14 October 2019 by Registry (Talk | contribs)

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Int7attB-BsaI sites-terminator-Int7attP

We expected that this part can be placed between a promoter and a translational unit part and work as a normally open (NO) switch for the downstreamed gene. and switch to ON state by excising the terminator ECK120033737 between the att sites when it reacted with Int7 integrase (BBa_K3254007). However, we haven't observed the expression of downstreamed gfp gene. That's may caused by the potential terminator activity of attL site after the recombination. Two BsaI restriction site were added between attB site and terminator. As a result, it can work as a normally closed (NC) switch for the gene which was inserted between the two BsaI site and switch to OFF state when it was excised.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 76
    Illegal BsaI.rc site found at 64


[edit]
Categories
//function/recombination
Parameters
None