Coding
XylR

Part:BBa_K143036:Design

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-09-16)
Revision as of 14:00, 17 September 2008 by Saint (Talk | contribs) (→‎Design Notes)

Xylose operon regulatory protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The XylR protein was identified in the genome using its Genbank entry[2] and NCBI's sequence viewer and PCR primers designed from the sequence. Biobrick prefix and suffix sequences were added and the gene cloned by PCR with Pfu DNA polymerase

Source

The XylR protein was PCR cloned form the B. subtilis genome using Pfu DNA polymerase

References

<biblio>

  1. 1 pmid=2544559
  2. 2 http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene, Gene ID:939531

</biblio>