Coding

Part:BBa_K3140002

Designed by: Fahad Ali   Group: iGEM19_Sydney_Australia   (2019-10-12)
Revision as of 01:20, 14 October 2019 by Fahad3li (Talk | contribs)


PsiK - 4-hydroxytryptamine kinase from Psilocybe cubensis

PsiK is a 4-hydroxytryptamine kinase, which catalyses the conversion of 4-hydroxytryptamine to norbaeocystin.

Usage and Biology

PsiK is a native enzyme obtained from Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both 4-hydroxytryptamine and psilocin as substrates to yield norbaeocystin and psilocybin, respectively. Heterologous expression of PsiK has been achieved in Escherichia coli <add strain> in a <expression system>.

ADD: -SDS-PAGE - pCW - LC/MAS

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 864
    Illegal SapI.rc site found at 973


[edit]
Categories
Parameters
None