Composite
Part:BBa_K3147005:Design
Designed by: Thomas Bessede Group: iGEM19_Montpellier (2019-10-13)
TEV protease with TEV-cleavable maltose binding protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 379
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Note
The TEV protease is a highly specific cysteine protease of the tabacco ech virus sequence. The protease is highly specific for the consensus sequence for its cleavage site which is ENLYFQ\G (BBa_ J18918) with \ indicating cleaved peptide binding. The Terminator used is a double terminator (BBa_0015).