Reporter

Part:BBa_K3147004:Design

Designed by: Thomas Bessede   Group: iGEM19_Montpellier   (2019-10-13)
Revision as of 19:09, 13 October 2019 by ThomasBCD (Talk | contribs)

mRFP1 fused to a TEV-cleavable ssrA tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 691
  • 1000
    COMPATIBLE WITH RFC[1000]


Design note

The TEV cutting site used is the conventional site composed of 6 amino acids: ENLYFQS. We have observed that this construction is weakly expressed. We advise future users to add a RiboJ insulator and a BCD2 element to improve its expression.