Reporter
Part:BBa_K3147004:Design
Designed by: Thomas Bessede Group: iGEM19_Montpellier (2019-10-13)
mRFP1 fused to a TEV-cleavable ssrA tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 691
- 1000COMPATIBLE WITH RFC[1000]
Design note
The TEV cutting site used is the conventional site composed of 6 amino acids: ENLYFQS. We have observed that this construction is weakly expressed. We advise future users to add a RiboJ insulator and a BCD2 element to improve its expression.