Composite

Part:BBa_K3142002:Design

Designed by: Luo Xiaolin   Group: iGEM19_SZPT-CHINA   (2019-09-28)
Revision as of 19:08, 13 October 2019 by Xiaolin (Talk | contribs) (→‎Source)

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Acid-induced promoter and repressor lacR of lac operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal NgoMIV site found at 149
    Illegal NgoMIV site found at 1052
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 989
    Illegal SapI.rc site found at 718


Design Notes

It only applies to lactic acid bacteria


Source

Lactobacillus delbrueckii subsp. Lactis NCC936

References