Composite

Part:BBa_K3142002

Designed by: Luo Xiaolin   Group: iGEM19_SZPT-CHINA   (2019-09-28)
Revision as of 19:05, 13 October 2019 by Xiaolin (Talk | contribs)


Acid-induced promoter and repressor lacR of lac operon

what it is：Lac repressor (lacR) and operator sequences (O1 and O2) is part of the lactose operon derived from Lactobacillus delbrueckii. O1 and O2 may serve as operators for the binding of the lac repressor (lacR).

what it does：O1 and O2 may serve as operators for the binding of the lac repressor (LacR) to prevent repressing the expression of downstream genes.

how to use it in other projects：BBa_K609001. When the external pH value declines to 5.5, rcfB promoter will be highly upregulated. Along with that, the lacR gene, which is downstream of rcfB will be highly transcribed. The large translation of LacR, the repressor of lac operon in Lactobacillus delbrueckii, will inhibit the use of lactose in the bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal NotI site found at 15
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 9
    Illegal XbaI site found at 24
    Illegal NgoMIV site found at 149
    Illegal NgoMIV site found at 1052
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 989
    Illegal SapI.rc site found at 718


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Parameters
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