Coding

Part:BBa_K3019012:Design

Designed by: Valeriia Leoshko   Group: iGEM19_Tartu_TUIT   (2019-10-13)
Revision as of 18:02, 13 October 2019 by Valeriia (Talk | contribs) (→‎Design Notes)

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α-factor pre-pro signal BBa_K2711000


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 517
    Illegal BamHI site found at 737
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 406
    Illegal NgoMIV site found at 768
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Illegal cutting sites removal

Source

BBa_K2711000 sequence is taken from iGEM registry, Alfa factor pre-pro signal sequence was taken from yeast genomic DNA.

References