Coding
LacI

Part:BBa_K143033:Design

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-09-16)
Revision as of 20:43, 16 September 2008 by Chris (Talk | contribs) (→‎References)

LacI (Lva-, N-terminal deletion) regulatory protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LacI was located in the sequence of the B. subtilis shuttle vector pDR111. This version of LacI lacks a Ltva degradation sequence and has a small N-terminal deletion that is observed in many LacI used in studies on B.subtilis. In particular, this LacI protein is used in pDR111 to regulate expression of the inducible Phyper-spank protein (BBa_K143015) (also used in the pDR111 vector). The BioBrick prefix and suffix were applied to the gene


Source

The LacI gene was cloned fromB. subtilis shuttle vector pDR111 using Pfu DNA polymerase PCR

References

<biblio>

  1. 1 pmid=16166525

</biblio>