Coding
nuclease_y

Part:BBa_K3027002

Designed by: Arnaud Boudigou   Group: iGEM19_GO_Paris-Saclay   (2019-09-24)
Revision as of 16:06, 13 October 2019 by Arnaud-Boudigou (Talk | contribs)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1235
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Nuclease_gp3 sequence (531 amino acids):

Met Lys Val Phe Glu Ala Lys Thr Leu Leu Thr Glu Ala Glu Lys Arg Ala Gln Glu Tyr Lys Asp Leu Lys Ser Lys Met Val Lys Leu Lys Lys Ala Phe Lys Ala Val Ala Asp Leu Asp Asp Ser Glu Phe Ser Gly Lys Gly Ala Asn Asn Ile Lys Ser Phe Tyr Glu Asp Gln Ala Gly Ile Ala Asp Gln Trp Ile Asp Leu Ile Glu Met Lys Ile Ser Phe Leu Thr Ser Ile Pro Gly Phe Leu Glu Asp Ala Asn Leu Ser Asp Ala Tyr Ile Glu Glu Thr Phe Leu Ala His Glu Leu Ala Asn Ala Tyr Thr Lys Ser Lys Ser Ile Met Ser Glu Gln Lys Lys Ala Met Lys Asp Ile Leu Asn Asp Ile Asn Asp Ile Leu Pro Leu Asp Leu Phe Ser Thr Glu Thr Phe Lys Asn Glu Leu Ser Ser Ala Glu Lys Lys Arg Lys Glu Ala Ile Glu Lys Met Asp Glu Val Asp Gln Asn Leu Thr Ser Glu Tyr Gly Leu Ser Glu Ala Asn Glu Gln Met Ile Gln Ala Asp Tyr Gln Ala Leu Met Asn Ala Thr Ala Lys Gly Lys Ser Ala Ser Pro Ile His Tyr Asn Ala Lys Ala Tyr Arg Asp Ser Glu Ile His Lys Met Thr Glu Asp Val Lys Lys Gln Ser Thr Asp Tyr Ile Ser Phe Lys Asp Gln Gln Ala Glu Gln Arg Arg Ile Ala Lys Glu Gln Glu Glu Leu Ala Asn Arg Pro Trp Tyr Glu Lys Ser Trp Asp Ala Val Cys Asn Phe Thr Gly Glu Val Ser Gly Tyr Tyr Asp Tyr Lys Arg Ala Ala Asp Gly Val Asp Pro Val Thr Gly Glu Lys Leu Thr Ala Gly Gln Arg Val Ala Ala Gly Ala Met Ala Ala Ala Gly Tyr Ile Pro Ile Val Gly Trp Ala Gly Lys Leu Ala Lys Gly Gly Lys Ala Val Tyr Ser Thr Ser Lys Ala Leu Tyr Arg Ala Asp Lys Ala Leu Asp Val Tyr Lys Thr Pro Lys Thr Phe His Ala Leu Gln Asn Ser Ser Lys Gly Leu Tyr Gly Leu Ala Ser Ala Asn Gly Phe Ser Glu Ala Ile Thr Gly Arg Asp Met Phe Gly Asn Lys Val Ser Lys Glu Arg Gln Glu Gln Ser Leu Ser Gly Ala Met Ala Met Leu Val Pro Phe Gly Ala Arg Gly Ile Asn Lys Lys Leu Asn Ala Lys Ser Ser Ser Arg Val Ser Glu Ala Ser Thr Asn Thr Ser Lys Lys Pro Lys Val Pro Lys Thr Tyr Lys Arg Pro Thr Tyr Phe Arg Lys Gly Val Arg Asp Lys Val Trp Glu Asn Ala Lys Asp Ser Thr Gly Ser Val Lys Asp Pro Leu Thr Lys Gln Val Met Lys Lys Asp Glu Pro Trp Asp Met Gly His Lys Pro Gly Tyr Glu Phe Arg Lys His Gln Gln Ser Ala Met Glu Arg Asn Ile Ser Arg Lys Gln Phe Leu Asp Glu His Asn Asn Pro Asp His Tyr Gln Pro Glu Leu Pro Ser Ser Asn Arg Ser His Lys Gly Glu Asp Met Thr Asp Asp Tyr Phe Gly Asp

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Categories
Parameters
None