Composite

Part:BBa_K3052010

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-13)
Revision as of 16:05, 13 October 2019 by Registry (Talk | contribs)

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Precursor Circuit -- a series of enzymes used for GPP production

This circuit contains eight enzymes in plant's MVA pathway to produce geranyl pyrophosphate(GPP),a precursor to various metabolites unique in plants like linalool and limonene. All of the genes in this circuit are inducible by IPTG since all of them are under control of Ptrc or Plac.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1557
    Illegal XbaI site found at 8869
    Illegal SpeI site found at 10020
    Illegal PstI site found at 4253
    Illegal PstI site found at 6546
    Illegal PstI site found at 6837
    Illegal PstI site found at 7196
    Illegal PstI site found at 11005
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1557
    Illegal NheI site found at 3467
    Illegal SpeI site found at 10020
    Illegal PstI site found at 4253
    Illegal PstI site found at 6546
    Illegal PstI site found at 6837
    Illegal PstI site found at 7196
    Illegal PstI site found at 11005
    Illegal NotI site found at 7688
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1557
    Illegal BglII site found at 1566
    Illegal XhoI site found at 11386
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1557
    Illegal XbaI site found at 8869
    Illegal SpeI site found at 10020
    Illegal PstI site found at 4253
    Illegal PstI site found at 6546
    Illegal PstI site found at 6837
    Illegal PstI site found at 7196
    Illegal PstI site found at 11005
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1557
    Illegal XbaI site found at 8869
    Illegal SpeI site found at 10020
    Illegal PstI site found at 4253
    Illegal PstI site found at 6546
    Illegal PstI site found at 6837
    Illegal PstI site found at 7196
    Illegal PstI site found at 11005
    Illegal NgoMIV site found at 1443
    Illegal NgoMIV site found at 5780
    Illegal NgoMIV site found at 7726
    Illegal AgeI site found at 8179
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None