![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3052010
Designed by: Ma WenHe Group: iGEM19_XJTU-CHINA (2019-10-13)
Precursor Circuit -- a series of enzymes used for GPP production
This circuit contains eight enzymes in plant's MVA pathway to produce geranyl pyrophosphate(GPP),a precursor to various metabolites unique in plants like linalool and limonene. All of the genes in this circuit are inducible by IPTG since all of them are under control of Ptrc or Plac.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1557
Illegal XbaI site found at 8869
Illegal SpeI site found at 10020
Illegal PstI site found at 4253
Illegal PstI site found at 6546
Illegal PstI site found at 6837
Illegal PstI site found at 7196
Illegal PstI site found at 11005 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1557
Illegal NheI site found at 3467
Illegal SpeI site found at 10020
Illegal PstI site found at 4253
Illegal PstI site found at 6546
Illegal PstI site found at 6837
Illegal PstI site found at 7196
Illegal PstI site found at 11005
Illegal NotI site found at 7688 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1557
Illegal BglII site found at 1566
Illegal XhoI site found at 11386 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1557
Illegal XbaI site found at 8869
Illegal SpeI site found at 10020
Illegal PstI site found at 4253
Illegal PstI site found at 6546
Illegal PstI site found at 6837
Illegal PstI site found at 7196
Illegal PstI site found at 11005 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1557
Illegal XbaI site found at 8869
Illegal SpeI site found at 10020
Illegal PstI site found at 4253
Illegal PstI site found at 6546
Illegal PstI site found at 6837
Illegal PstI site found at 7196
Illegal PstI site found at 11005
Illegal NgoMIV site found at 1443
Illegal NgoMIV site found at 5780
Illegal NgoMIV site found at 7726
Illegal AgeI site found at 8179 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |