Reporter

Part:BBa_K3147003

Designed by: Thomas Bessede   Group: iGEM19_Montpellier   (2019-10-13)
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mRFP1 fused to a TEV-cleavable ssrA tag

I : parts BBa_K3147003 (mRFP1-TEVcs-SSRA) function : Short description : mRFP1 with degradation tag that can be separated by TEV protease The Montpellier 2019 team made a reporter gene construction in order to carry out their proof of concept for the project. This construction produces an mRFP1 (BBA_E1010) fused in C-ter with a rapid degradation tag of SSRA (BBA_M0050) type . The TEV cutting site (BBa_J18918) was added between the mRFP1 and the SSRA tag. A double terminator controls the expression of this parts (BBa_B0015).

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Figure 1: Construct Design: mRFP1 fused to an SSRA proteolysis tag with a TEV cutting site between the two. This construction can be used as a reporter gene, in the presence of TEV the SSRA is separated and allows the mRFP to glow.

The objective of the construction was to be able to confirm that a TEV merged with an anti-SfGFP VHH is specific to the SfGFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 691
  • 1000
    COMPATIBLE WITH RFC[1000]


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