Generator

Part:BBa_K3187039

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-13)
Revision as of 15:25, 13 October 2019 by Registry (Talk | contribs)

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tetR/tetA promotor, RBS, mCherry and T7 terminator

This part includes the tetR/tetA promotor + tet operator which can be induced by the addition of anhydrotetracycline (aHT) and a ribosomal binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
    Illegal PstI site found at 805
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
    Illegal NheI site found at 810
    Illegal PstI site found at 805
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
    Illegal PstI site found at 805
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
    Illegal PstI site found at 805
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None