Composite
Part:BBa_K2923020
Designed by: Kateryna LEN Group: iGEM19_Strasbourg (2019-10-13)
MS2-MS2-Guanine actif aptazyme-PP7-PP7
Our bacterial three-hybrid (BH3) construct (inspired by Berry and Hochschild, 2018) is composed of two fusion proteins with MS2 and PP7 coat protein and RNA. To make the interaction between BH3 and guanine aptazyme iGEM Strasbourg created an aptazyme bordered by MS2 and PP7 stem-loops. This aptazyme is specific for the guanine or guanosine ligand. It is composed of 3 different parts: a ribozyme module, a communication module, and an aptamer module. This aptazyme is an ON-switches type of aptazyme. Its catalytic activity (self-cleavage) requires the presence of ligand.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 25
Illegal PstI site found at 64 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 37
Illegal PstI site found at 25
Illegal PstI site found at 64 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//classic/rna/uncategorized
ligands | in-vitro assay : Guanine; in-vivo assay Guanosine |
switch_point | The maximum effect obtained at approximately 500 μM of guanosine |