Coding

Part:BBa_K3019011

Designed by: Valeriia Leoshko   Group: iGEM19_Tartu_TUIT   (2019-10-13)
Revision as of 14:16, 13 October 2019 by Registry (Talk | contribs)

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α-factor pre-pro signal GLCI

Alfa-factor pre-pro pheromone signal is the most widely used secretion signal in yeast, which contains two regions: a 19-amino acid N-terminal signal sequence (pre-region) that directs translocation into the endoplasmic reticulum (ER), followed by a 66-amino acid pro region that mediates packaging into ER-derived transport vesicles. Arthrobacter sp. YCWD3 glcI gene for beta-1,3-glucanase, complete cds. Codon optimised for expression in S. cerevisiae. This glucanase is a part of Zymolyaze.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 549
    Illegal AgeI site found at 1630
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None