Composite

Part:BBa_K2963032

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-19)
Revision as of 13:43, 13 October 2019 by GHX (Talk | contribs)


racE- encoding racemase

This part contains the ""racE"" racemase gene, which was constructed using the tac (BBa_K864400) promoter, rbs (BBa_K2963006), racE gene (BBa_K2963004) and the T7 terminator (BBa_K2598024). This part was compared with the other part (BBa_K2963033) which contained another lac operator follwowing the tac promoter（BBa_K864400）.Simultaneously, the rest basic parts are the same. The racemase activity data and real-time PCR data were used to characterize the composite part. And we compared the different expression levels of racE gene of the two composite (BBa_K2963033 and BBa_K2963032）. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 911
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters

[edit]
Categories
Parameters
None