Composite

Part:BBa_K3286003

Designed by: Paul Alexander Niederau   Group: iGEM19_Wageningen_UR   (2019-10-13)
Revision as of 13:34, 13 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


sgRNA expression module

Expression cassette of the sgRNA with non-targeting (NT) spacer (https://parts.igem.org/Part:BBa_K3286001) and rho-independent terminator (https://parts.igem.org/Part:BBa_K3286002) under control of the strong constitutive Anderson promoter J23109 (https://parts.igem.org/Part:BBa_J23119).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None