Regulatory

Part:BBa_K2947002

Designed by: Shan Huang   Group: iGEM19_GDSYZX   (2019-10-13)
Revision as of 12:34, 13 October 2019 by Shadowburn (Talk | contribs)


d35S

d35S refers to Double GoldenGate compatible 35S Promoter. 35S is a plant specific promoter obtained from the Cauliflower Mosaic Virus. We add two 35s promotor together to get a d35s promotor, then we merge GFP gene with d35s promotor to make a binary expression vector. After that, we extract plasmids, transfer the plasmids into the protoplasts of Arabidopsis thaliana and culture the protoplasts for 12 hours to view fluorescence intensity run protein gel electrophoresis and Western blot to test the GFP expression rate.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None