Composite

Part:BBa_K2922020

Designed by: Shuling Xiao   Group: iGEM19_XMU-China   (2019-10-13)
Revision as of 12:10, 13 October 2019 by Registry (Talk | contribs)

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T7-RBS-cex expressing Exoglucanase functions in Kil secretion cassette with promoter J23114

This part contains the sequence for the protein kil regulated by constitutive promoter J23114 and the sequence for the protein exoglucanase regulated by T7 promoter. We used this part to achieve the secretion of exoglucanase with the function of kil secretion cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 818
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 451
    Illegal NgoMIV site found at 824
    Illegal NgoMIV site found at 1326
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 871
    Illegal SapI.rc site found at 954


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Parameters
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