Composite
Part:BBa_K2922013
Designed by: Jisheng Xie Group: iGEM19_XMU-China (2019-10-13)
T7-RBS-bgl1A (Beta-D-glucosidase) functions in Kil secretion cassette with promoter J23112
This part contains the sequence for the protein kil regulated by constitutive promoter J23112 and the sequence for the protein beta-D-glucosidase regulated by T7 promoter. We used this part to achieve the secretion of endoglucanase with the function of kil secretion cassette.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1507
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |