Composite

Part:BBa_K2922012

Designed by: Jisheng Xie   Group: iGEM19_XMU-China   (2019-10-13)
Revision as of 11:58, 13 October 2019 by Registry (Talk | contribs)

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T7-RBS-bgl1A (Beta-D-glucosidase) functions in Kil secretion cassette with promoter J23109

This part contains the sequence for the protein kil regulated by constitutive promoter J23109 and the sequence for the protein beta-D-glucosidase regulated by T7 promoter. We used this part to achieve the secretion of endoglucanase with the function of kil secretion cassette.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1507
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None