Project

Part:BBa_K3113302

Designed by: Alejandro Salinas Illarena   Group: iGEM19_Munich   (2019-10-12)
Revision as of 11:51, 13 October 2019 by Theresakeil (Talk | contribs)


pCAG_Gag-HiBiT-L7Ae

This plasmid codes for the coat protein of HIV fused to a split luciferase fused to a synthetic L7Ae. This construct forms virus-like particles which can be detected via a split luciferase assay. It is used to target fluc specifically into exosomes via L7Ae-C/Dbox connection.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3019
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2319


[edit]
Categories
//awards/composite_part/nominee
Parameters
None