Composite

Part:BBa_K2922010

Designed by: Fude Chen   Group: iGEM19_XMU-China   (2019-10-13)
Revision as of 11:41, 13 October 2019 by Registry (Talk | contribs)

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Constitutive promoter J23112, strong RBS and Kil protein combination for high secretion levels

Kil protein accumulates in the periplasmic space of the bacteria, the periplasmic space was increased and the membrane permeability of bacteria was improved, thereby the protein inside the bacteria can secrete out of bacteria. But only when the intensity of the promoter is proper, the secretion can be achieved and prevent cell lysis and death. Here we use different constitutive promoters to regulate the expression of kil protein to enhance its secretion ability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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