Tag
Part:BBa_K3002017
Designed by: Dorothée Klein Group: iGEM19_TU_Kaiserslautern (2019-10-13)
3xHA tag for Chlamydomonas reinhardtii (Phytobrick)
This basic part contains the sequence of the 3xHA-tag (B5) and was built as a part of the Kaiser Collection. Combined with a promoter (e.g. BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))), a CDS and terminator (e.g. BBa_K3002006 (RPL23 terminator)) your target protein can be detected via HA-antibody.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |