Coding

Part:BBa_K3002029:Design

Designed by: Marlene Schlosser   Group: iGEM19_TU_Kaiserslautern   (2019-10-13)
Revision as of 10:01, 13 October 2019 by MSchlosser (Talk | contribs) (→‎Design Notes)


Wildtype MHETase for Chlamydomonas reinhardtii Part 2 (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 57
    Illegal PstI site found at 331
    Illegal PstI site found at 1141
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 57
    Illegal PstI site found at 331
    Illegal PstI site found at 1141
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 909
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 57
    Illegal PstI site found at 331
    Illegal PstI site found at 1141
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 57
    Illegal PstI site found at 331
    Illegal PstI site found at 1141
    Illegal NgoMIV site found at 403
    Illegal NgoMIV site found at 421
    Illegal NgoMIV site found at 457
    Illegal NgoMIV site found at 1100
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The MHETase gene was splitted into two pieces in order to codon-optimize it for Chlamydomonas reinhardtii. BBa_K3002005 is the first part of the MHETase and BBa_K3002029 is the second part. Additionally, this part is compatible with Phytobricks /MoClo parts.

Source

This part originates from Ideonella sakaiensis (Yoshida, S., 2016).


References