Primer

Part:BBa_K3253001

Designed by: HAOYU JIANG   Group: iGEM19_Nanjing   (2019-10-12)
Revision as of 02:58, 13 October 2019 by Registry (Talk | contribs)

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Gene-EcoR1-F

this is the primer we added to the gene in order to make it be successfully cut by the EcoR1 restriction enzyme

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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