Regulatory

Part:BBa_K2983051

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-12)
Revision as of 22:17, 12 October 2019 by TomZaplana (Talk | contribs)


pTef1d: wild-type without the BsaI site

This part is a version of the Yarrowia lipolytica pTef1 promoter, derived from the wild type pTef1 promoter (nucleotides 1227374 to 1226969) of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1).

It is an improvement of BBa_K2117000 as it has a mutated BsaI site (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 199
    Illegal PstI site found at 164
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None