Regulatory
Part:BBa_K2983051
Designed by: Tom Zaplana Group: iGEM19_Evry_Paris-Saclay (2019-10-12)
Revision as of 22:17, 12 October 2019 by TomZaplana (Talk | contribs)
pTef1d: wild-type without the BsaI site
This part is a version of the Yarrowia lipolytica pTef1 promoter, derived from the wild type pTef1 promoter (nucleotides 1227374 to 1226969) of Yarrowia lipolytica W29 chromosome C (GenBank Acc. n° CP028450.1).
It is an improvement of BBa_K2117000 as it has a mutated BsaI site (GGTCTC to GGTCTg) which makes this part compatible with the iGEM Type IIS RFC[1000] standard.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 199
Illegal PstI site found at 164 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 199
Illegal PstI site found at 164 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 199
Illegal PstI site found at 164 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 199
Illegal PstI site found at 164 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |