Regulatory

Part:BBa_K2983052

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-12)
Revision as of 22:13, 12 October 2019 by Registry (Talk | contribs)

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pTef1e: wild-type without the BsaI site and shorter

This part is a version of the Yarrowia lipolytica pTef1 promoter, derived from the Addgene plasmid #120738.

It is an improvement of BBa_K2117000 as it is shorter and does not have the BsaI site which makes this part compatible with the iGEM Type IIS RFC[1000] standard.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 192
    Illegal PstI site found at 157
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None