Coding

Part:BBa_K3190101

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-12)
Revision as of 22:04, 12 October 2019 by Registry (Talk | contribs)

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G protein-coupled estrogen receptor (GPER/GPR30) CDS


G protein-coupled estrogen receptor (GPR30, also referred to as GPER), an intracellular transmembrane estrogen receptor, was identified in 2005 (Revankar, 2005). It is found to localise to the endoplasmic reticulum and specifically binds to estrogen and its derivatives (the ligand). The interaction between estradiol and the membrane-associated receptor triggers non-genomic signalling; intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. The gene encoding for the receptor was codon optimised and coupled to the strongest constitutive promoter pCCW12 for heterologous expression in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 750


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Parameters
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