Part:BBa_K2984006
Codon-Usage-Optimized Paromomycin Resistance For Use in Chlamydomonas reinhardtii
Paromomycin belongs to a group of aminoglycoside antibiotics such as neomycin or dibekacin. These aminoglycosides are capable of inhibiting the eukaryotic translation, by binding within the large and small subunit of the 80S ribosome. This property allows paromomycin to be used as a selection marker. The paromomycin resistance BBa_K2703008, registered by the Cambridge team 2016, works by allowing the expression of an enzyme which catalyses the transfer of the gamma-phosphate of ATP to the hydroxyl group in 3’ position of the paromomycin molecule and allows the carrier of the gene to develop a resistance to paromomycin. Some organisms exhibit a higher expression of transgene enzymes when the genetic code is codon optimized for the specific organism. One of these organisms is the alga Chlamydomonas reinhardtii. With the following part, we propose a codon optimized version of the paromomycin resistance for use in Chlamydomonas reinhardtii. This part is optimal for paromomycin screening when using Ch Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 298
Illegal NotI site found at 122 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 13
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 401
- 1000COMPATIBLE WITH RFC[1000]
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