Part:BBa_K3002005
Wildtype MHETase for Chlamydomonas reinhardtii Part 1 (Phytobrick)
This basic part contains the coding sequence of the wildtype MHETase (B3) and was built as a part of the Kaiser Collection. This part is codon-optimized for Chlamydomonas reinhardtii. Combined with the second part of the MHETase (BBa_K3002029), a promoter and a terminator, this level 0 construct mediates PET degradation ability. As this part contains the introns 1 and 2 of RBCS2, it perfectly matches the part BBa_K3002027 (pAR promoter A1-B2), resulting in a high expression (Eichler-Stahlberg et al., 2009). To detect or purify the target protein a tag of the Kaiser Collection like BBa_K3002010 (sp20 HA-tag), BBa_K3002017 HA-tag), BBa_K3002018 (sp20 His-tag), BBa_K3002028 (His-tag) is recommended. A secretion signal (BBa_K3002007 (cCA), BBa_K3002008 (GLE) or BBa_K3002009 (ARS)) can be added, when using the part BBa_K3002003 (pAR promoter (A1-A3)) as a promoter.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 711
Illegal PstI site found at 1089 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 711
Illegal PstI site found at 1089
Illegal NotI site found at 1100 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 711
Illegal PstI site found at 1089 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 711
Illegal PstI site found at 1089
Illegal NgoMIV site found at 1027
Illegal NgoMIV site found at 1159
Illegal NgoMIV site found at 1189 - 1000COMPATIBLE WITH RFC[1000]
None |