Coding

Part:BBa_K3002005:Design

Designed by: Dorothée Klein   Group: iGEM19_TU_Kaiserslautern   (2019-10-12)
Revision as of 21:11, 12 October 2019 by Kleind (Talk | contribs)


Wildtype MHETase for Chlamydomonas reinhardtii Part 1 (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 711
    Illegal PstI site found at 1089
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 711
    Illegal PstI site found at 1089
    Illegal NotI site found at 1100
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 711
    Illegal PstI site found at 1089
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 711
    Illegal PstI site found at 1089
    Illegal NgoMIV site found at 1027
    Illegal NgoMIV site found at 1159
    Illegal NgoMIV site found at 1189
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The MHETase gene was splitted into two pieces in order to codon-optimize it for Chlamydomonas reinhardtii. <a href="https://parts.igem.org/Part:BBa_K3002005">BBa_K3002005</a> is the first part of the MHETase and <a href="https://parts.igem.org/Part:BBa_K3002029">BBa_K3002029</a> is the second part. Additionally, this part is compatible with Phytobricks /MoClo parts.


Source

This part originates from Ideonella sakaiensis (<a href="https://science.sciencemag.org/content/351/6278/1196.full">Yoshida, S., 2016</a>).

References