Composite

Part:BBa_K3105685

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-12)
Revision as of 20:46, 12 October 2019 by Registry (Talk | contribs)

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Expression construct AAO

Aryl-alcohol oxidase (AAO) cloned in-frame with α-factor from Saccharomyces cerevisiae. This construct is controlled by the AOX1 promotor, which is inducible by methanol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1356
    Illegal BamHI site found at 1543
    Illegal XhoI site found at 1183
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2503
    Illegal AgeI site found at 3116
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None