Composite
Part:BBa_K2959010:Design
Designed by: Alejandro Aguirre Hernndez Group: iGEM19_Tec-Chihuahua (2019-10-12)
Expressible Arabidopsis thaliana Profilin 1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of AtPFN1 is succeeded by a 6x His-Tag to facilitate its purification by immobilized metal affinity chromatography.
Source
The composite part was designed using the following parts previously added to the Registry: BBa_K525998, and BBa_B0015. The amino acid sequence for AtPFN1 was retrieved from Christensen et al. (1996).