Coding

Part:BBa_K3037001:Design

Designed by: Arnau Pérez Roig   Group: iGEM19_TU_Dresden   (2019-10-03)
Revision as of 18:59, 12 October 2019 by ArnauP (Talk | contribs)


Maltose Binding Protein (MBP-tag)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 381
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 79


Design Notes

Prescission site upstream and downstream.

Biobrick used in RFC 25 standard

Codon optimized for E. coli with all forbidden restriction enzyme sites removed


Source

Synthesized by Integrated DNA Technologies (IDT)

References