Coding

Part:BBa_K3102011:Design

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-03)
Revision as of 17:00, 12 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎References)


EG1 (Endo-1,4-beta-glucanase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1112
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
    Illegal AgeI site found at 313
    Illegal AgeI site found at 403
    Illegal AgeI site found at 1240
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence synthesized and optimized for E.coli on Geneius



Source

Neisseria Sicca, sequence found through genebank https://www.ncbi.nlm.nih.gov/protein/BAJ21449.1

References

Moriyoshi K, Ohmoto T, Ohe T, Sakai K. "Role of Endo-1,4-β-glucanases from Neisseria sicca SB in synergistic degradation of cellulose acetate". Biosci Biotechnol Biochem. 2003;67(2):250–7.