Coding

Part:BBa_K3102010:Design

Designed by: Chloe DOIZELET   Group: iGEM19_Ionis_Paris   (2019-10-03)
Revision as of 17:00, 12 October 2019 by ChloeDOIZELET (Talk | contribs) (→‎References)


CAE (cellulose acetate esterase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 75
    Illegal NgoMIV site found at 1185
    Illegal AgeI site found at 205
    Illegal AgeI site found at 292
    Illegal AgeI site found at 430
    Illegal AgeI site found at 613
    Illegal AgeI site found at 661
    Illegal AgeI site found at 829
    Illegal AgeI site found at 1042
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence synthesized and optimized for E.coli on Geneius


Source

Neisseria Sicca, sequence found through genebank : https://www.ncbi.nlm.nih.gov/protein/BAJ21448.1

References

Moriyoshi K, Ohmoto T, Ohe T, Sakai K. "Purification and characterization of an esterase involved in cellulose acetate degradation by neisseria sicca SB". Biosci Biotechnol Biochem. 1999;63(10):1708–13.

Moriyoshi K, Koma D, Yamanaka H, Ohmoto T, Sakai K. "Functional analysis of the carbohydrate-binding module of an esterase from neisseria sicca SB involved in the degradation of cellulose acetate. Biosci Biotechnol" Biochem. 2010;74(9):1940–2.