Coding

Part:BBa_K3132101

Designed by: Qiliang Yang   Group: iGEM19_SMMU-China   (2019-10-12)
Revision as of 13:44, 12 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PIP_MinimalCMV Promotor

This part (8*PIP MinimalCPIP_MinimalCMVMV ) is one of our synthetic promoters (SynPro) based on minimalCMV promoter (BBa_K3132003) and eight repeats of PIP binding sites. We inset the 8*PIP binding sites before the minimalCMV promoter. MinimalCMV promoter is a promoter from the vector pcDNA3.1 with very strong transcriptional activity. In this way, we can use our synthetic transcription factors (SynTFs) which is a fusing protein PIP_DBD_(G4S) linker_NLS_VP64. The PIP-DBD can bind to the 8*PIP binding sites specifically and the VP64 domain can initiate transcription by activating the MinimalCMV 8*PIP promoter. The corresponding SynTF of PIP_minimalCMV is PIP-VP64 (BBa_K3132004).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None