Device

Part:BBa_K2938016:Experience

Designed by: Assaf Vital   Group: iGEM19_BGU_Israel   (2019-10-09)
Revision as of 10:27, 12 October 2019 by Assafi (Talk | contribs)

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Applications of BBa_K2938016

In our plasmids, we placed the deGFP closer to the terminator. The presence of the GFP signal proves the expression of the previous subunits on the plasmid and it allows the detection of the transgenic bacteria easily (in mosquitoes)

Here we can see the level of fluorescence of liquid starters from Serratia expressing the different type of plasmid we created. (BBa_K2938014, BBa_K2938015, BBa_K2938016).

Fluorescence levels of Serratia expressing our different plasmids

We made toxicity assays, In order to check how effective and toxic are our plasmids. We hatched mosquitoes untreated eggs in water containing Serratia bacteria expressing the different plasmids. Then we counted the live larva 48 hours after treatment.

Toxicity assay results, Our plasmids show larvicidal activity

User Reviews

UNIQ7b80ec4f0296c83b-partinfo-00000000-QINU UNIQ7b80ec4f0296c83b-partinfo-00000001-QINU