Coding
Part:BBa_K3102012
Designed by: Sarah DUQUESNE Group: iGEM19_Ionis_Paris (2019-10-03)
Revision as of 08:15, 12 October 2019 by ChloeDOIZELET (Talk | contribs)
TEV protease, the common name for the 27 kDa catalytic domain of the Nuclear Inclusion is a (NIa) protein encoded by the tobacco etch virus (TEV). Its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins.
Consequently, it’s an highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)(BBa_K3102041) and cleaves between the Gln and Gly/Ser residues.
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