Coding

Part:BBa_K3102012

Designed by: Sarah DUQUESNE   Group: iGEM19_Ionis_Paris   (2019-10-03)
Revision as of 08:15, 12 October 2019 by ChloeDOIZELET (Talk | contribs)

TEV protease, the common name for the 27 kDa catalytic domain of the Nuclear Inclusion is a (NIa) protein encoded by the tobacco etch virus (TEV). Its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins.

Consequently, it’s an highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)(BBa_K3102041) and cleaves between the Gln and Gly/Ser residues.

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