Coding

Part:BBa_K2963002:Design

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-06)
Revision as of 06:59, 12 October 2019 by GHX (Talk | contribs) (→‎Source)

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capC- coding a subunit of poly-γ-glutamic acid synthetase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 251
    Illegal EcoRI site found at 310
    Illegal NgoMIV site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we express the CapC protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.

Source

Derived from Bacillus licheniformis.

References