Coding

Part:BBa_K2963001:Design

Designed by: Experiment group members of JNU-China   Group: iGEM19_JNU-China   (2019-09-06)
Revision as of 06:58, 12 October 2019 by GHX (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


capA - encoding a subunit of poly-γ-glutamic acid synthetase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When we express the CapA protein heterologously in corynebacterium glutamicum, we add Ptac promoter and a short RBS, which can be recognized by corynebacterium glutamicum. And simultaneously, we add T7 terminater used for stopping transcription in time.


Source

derived from Bacillus licheniformis.

References