Regulatory
PdhP
Part:BBa_K3238000
Designed by: Irving Francisco Group: iGEM19_BUAP_Mexico (2019-10-12)
Pyruvate dehydrogenase complex promoter, pyruvate inducible
This part is the pyruvate inducible promoter controlling the pdhR-aceEF-lpdA operon of E. coli k-12 strain. This parts has showed regulation by pyruvate dehydrogenase complex repressor, however, this repressor could be inhibited by pyruvate. We characterized this part in E. coli strain BL21 to contribute with a new promoter that can be used to measure pyruvate production or activated by this molecule artificially.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |