Part:BBa_K3282000

This part contains coding sequence of Escherichia coli’s K12 (substr. MG1655) arsenical resistance operon repressor (arsR, Gene ID: 948013, Uniprot: P37309) codon-optimized for Escherichia coli Nissle 1917. The arsR gene which encodes for regulatory proteins, is a part of the ars operon of the E. coli conjugal plasmid R773 (1). ArsR is a 117 residue protein, that has been shown to be a trans‐acting repressor and is a sensor of environmental As(III). This 13 kDa protein belongs to the ArsR family of metalloregulatory proteins that respond to a variety of metals including As(III), Sb(III), Cd(II), and Zn(II) (2).

1. Carlin, A., Shi, W., Dey, S., Rosen, B. (1995). The ars operon of Escherichia coli confers arsenical and antimonial resistance. Journal of Bacteriology, 177 (4), 981-986; doi: 10.1128/jb.177.4.981-986.

2. Rastorguev, S.M., Zavilgelsky, G.B., Tchurikov, N.A. (1998). IncI1 plasmid R64 encodes the ArsR protein that alleviates type I restriction. FEBS Lett. 426, 21–23.