Coding

Part:BBa_K2915150

Designed by: SAADOUNI Rim   Group: iGEM19_Aix-Marseille   (2019-10-11)
Revision as of 16:43, 11 October 2019 by Registry (Talk | contribs)

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IPTG inducible promoter (T7) with RBS AFP-lu1 6-His-Tag with TEV site

The AFP-Iu1 it's a antifreeze which came from the Choristoneura fumiferana. These proteins lower the nonequilibrium freezing point of water while not affecting the melting point. The AFP proteins were used in our project in order to conserved all engeneering proteins we designed to create our TB diagnostic Kit.TheAFP protein can be purify because of the presence of a 6His-Tag in the N-terminal of the protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None