Coding

Part:BBa_K2915275:Design

Designed by: ARRINDELL Jennifer   Group: iGEM19_Aix-Marseille   (2019-10-11)
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IPTG inducible promoter (T7) with RBS TAL2 6-His-Tag with TEV site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 246


Design Notes

We add a 6Hig-Tag onto the "TAL2" sequence which allows us to purify our proteins. We optimized the codons to be able to produce these proteins into the K12 E.coli strain. To perform the production f TAL2 in a K12 E.coli strain, we added a T7 promotor and a RBS into the psb1c3 plasmide.



Source

Nucleotide sequence

References