Coding

Part:BBa_K3174008

Designed by: Kelsey Hu   Group: iGEM19_Austin_UTexas   (2019-10-09)
Revision as of 15:12, 11 October 2019 by Amacaskill (Talk | contribs)


SYFP2 coding sequence with IS hotspot removed

This is a redesigned version of the Super Yellow Fluorescent Protein 2 (BBa_K864100) that has had a region that was prone to insertion element mutations altered. This removal of a mutation hotspot resulted in the expression of SYFP2 in culture being maintained for a longer period of time than the original plasmid.


Usage and Biology

This part codes for the bright yellow fluorescent protein SYFP2, which has an excitation peak of 515 nm and an emission peak of 527 nm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None