Plasmid_Backbone
pSB1T3

Part:pSB1T3:Design

Designed by: Austin Che   Group: iGEM07_Example   (2008-09-08)
Revision as of 20:23, 8 September 2008 by Registry (Talk | contribs)

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High copy BioBrick assembly plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2440
    Illegal NheI site found at 1268
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2446
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2440
    Illegal BamHI site found at 1414
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 2316
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2440
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2440
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2455
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 1440
    Illegal NgoMIV site found at 1808
    Illegal NgoMIV site found at 1968
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The ampicillin resistance marker needed to be eliminated.


Source

Austin Che constructed this plamid from pSB1AT3-P1010.

References