Coding

Part:BBa_K3257044

Designed by: Yi Qiao   Group: iGEM19_Fudan-TSI   (2019-09-05)
Revision as of 12:05, 11 October 2019 by YY12138 (Talk | contribs)

Cre Recombinase

Cre recombinase is responsible for recombination of reverse transcribed cDNA, which is also one of the most important processes in our mutation library construction. We have expressed Cre recombinase in E.coli and proved its ability of gene cleavage when there are lox sites present.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 356
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 42
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None